期刊文献

Colorimetric liquid crystal-based assay for the ultrasensitive detection of AFB1 assisted with rolling circle amplification 收藏

比色液晶的测定法，用于超敏检测AFB1辅助滚动圆的扩增

原文求助发布源

作者

Wenli Wu[a][b][1];Shuang Xia[a][b][1];Mei Zhao[a][b];Jiantao Ping[a][b];Jin-Ming Lin[c];Qiongzheng Hu[a][b]

作者单位

[a]School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, ChinaSchool of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China[b]Qilu University of Technology (Shandong Academy of Sciences) , Shandong Analysis and Test Center, 19 Keyuan Street, Jinan 250014, ChinaQilu University of Technology (Shandong Academy of Sciences) ， Shandong Analysis and Test Center, 19 Keyuan Street, Jinan 250014, China[c]Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, ChinaBeijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China[1]1These authors contributed equally to the manuscript.

关键词

Liquid crystal;Aqueous microdroplets;Surfactant;Rolling circle amplification;Colorimetric assay;AFB1;

关键词译文

液晶;水晶微粒；表面活性剂；滚动圆扩增；比色测定； AFB1；

发布日期

8 August 2022

DOI

10.1016/j.aca.2022.340065

来源信息

Analytica Chimica Acta ISSN：0003-2670, 2022年, 1220卷

摘要

The detection of AFB1 that is a group I carcinogen is significantly important for food safety. Herein, we report a colorimetric liquid crystal (LC)-based assay that allows the ultrasensitive detection of AFB1. When an aqueous solution of a cationic surfactant is transferred onto the LCs dispersed with the aqueous microdroplets containing the anionic surfactants and horseperoxidase (HRP), it triggers the release of HRP due to the interfacial charge interaction. Because HRP can catalyze the colorless 3,3′-5,5′-tetramethylbenzidine (TMB) into yellow products, the response of the LCs dispersed with the aqueous microdroplets to the cationic surfactant is visually determined. In the presence of AFB1, the rolling circle amplification on magnetic beads (MBs) is triggered due to the specific recognition of AFB1 by its aptamer, which results in the generation of long chain single-stranded DNA on MBs. As the cationic surfactants are captured by the negatively charged ssDNA, it prevents the release of HRP into the aqueous solution. In contrast, in the absence of AFB1, HRP is released into the aqueous solution. The developed AFB1 sensing assay shows very good linear relationship with the detection limit of AFB1 determined to be as low as 0.014 pg/mL. In addition, the detection of AFB1 in rice and peanut oil is also examined to demonstrate its capability for the analysis of the real samples. Overall, this method takes advantages of the unique aptamer/target recognition, specific enzymatic reaction, and simple colorimetric assay, which makes it very promising for the ultrasensitive detection of AFB1 in practical applications.

摘要译文

AFB1的检测是I组致癌物，对于食品安全至关重要。本文中，我们报告了一个基于比色的液晶（LC）测定法，该测定法可以超敏检测AFB1。当将阳离子表面活性剂的水溶液转移到与含有阴离子表面活性剂和马过氧化物酶（HRP）水的微螺旋体分散的LCS上时，它会触发由于界面电荷相互作用而引起的HRP释放。由于HRP可以催化无色的3,3'-5,5'-四甲基苯胺（TMB）在黄产物中，因此在视觉上确定了与水性微孔分散的LCS对阳离子表面活性剂的反应。在存在AFB1的情况下，由于其适体对AFB1的特定识别而触发了磁珠（MB）上的滚动圆扩增，从而导致长链单链DNA在MBS上产生。由于阳离子表面活性剂是由带负电荷的ssDNA捕获的，因此可以防止HRP释放到水溶液中。相反，在没有AFB1的情况下，HRP被释放到水溶液中。开发的AFB1传感测定法显示出非常良好的线性关系，而确定低至0.014 pg/mL的AFB1的检测极限。此外，还检查了大米和花生油中AFB1的检测，以证明其对真实样品进行分析的能力。总体而言，该方法具有独特的适体/靶标识别，特定的酶促反应和简单的比色测定的优势，这使得在实际应用中对AFB1的超敏感检测非常有前途。

Wenli Wu[a][b][1];Shuang Xia[a][b][1];Mei Zhao[a][b];Jiantao Ping[a][b];Jin-Ming Lin[c];Qiongzheng Hu[a][b]. Colorimetric liquid crystal-based assay for the ultrasensitive detection of AFB1 assisted with rolling circle amplification[J]. Analytica Chimica Acta, 2022,1220