摘要
Summer savory (Satureja hortensis L.) is a medicinal and aromatic plant of the Lamiaceae family, a source of valuable secondary metabolites (monoterpenoids, rosmarinic acid, flavonoids). For this paper, flavonoid accumulation in an aseptic culture of summer savory was determined by using a colorimetric method. The organ specificity of flavonoid accumulation in aseptic plants was revealed: In leaves (8.35 ± 0.17 mg/g FW), flower buds (7.55 ± 0.29 mg/g FW), and calyx (5.27 ± 0.28 mg/g FW), flavonoids accumulated in significantly higher amounts than in stems (1.50 ± 0.22 mg/g FW) and corolla (0.78 ± 0.12 mg/g FW). We found that primary callus tissue formed from cotyledon and hypocotyl explants retains the ability to synthesize flavonoids at deficient levels (0.50 ± 0.09 mg/g FW and 0.44 ± 0.11 mg/g FW, respectively), that remained stable throughout six subcultures. Placing the callus tissue in monochrome lighting conditions with blue, green, and red light-emitting diode (LED) lamps leads to morphological changes in the tissue and decreased flavonoid accumulation compared to fluorescent lamps.
摘要译文
夏季咸味（Sateryja hortensis L.）是Lamiaceae家族的药用和芳香植物，这是有价值的次级代谢物（单甲基苯甲酸酯，粘胶酸，类黄酮）的来源。对于本文，使用比色法确定了夏季咸味的无菌培养物中类黄酮的积累。揭示了无菌植物中类黄酮积聚的器官特异性：在叶片（8.35±0.17 mg/g fw）中，花蕾（7.55±0.29 mg/g fw）和花萼（5.27±0.28 mg/g fw），类黄酮积累在明显高于茎（1.50±0.22 mg/g FW）和花冠（0.78±0.12 mg/g FW）中的量。我们发现，由子叶和下胚基外植体形成的原发性愈伤组织组织保持在缺乏水平（0.50±0.09 mg/g FW和0.44±0.11 mg/g FW）中合成类黄酮的能力，这些能力在整个六个亚文化中保持稳定。与荧光灯相比，将愈伤组织组织放置在蓝色，绿色和发射二极管（LED）灯的单色照明条件下，导致组织形态变化和黄酮类似降低。
Darya A. Khlebnikova;Evgeniya M. Efanova;Nina A. Danilova;Yaroslava V. Shcherbakova;Irina Rivera Sidorova. Flavonoid Accumulation in an Aseptic Culture of Summer Savory (Satureja hortensis L.)[J]. Plants, 2022,11(4): 533