摘要
Cumulus granulosa cells (CGCs) are a type of important ovarian somatic cells that carries out various functions related to oogenesis, follicular development and embryogenesis. The study on the development and function of CGCs facilitates the understanding of reproductive regulation in female animals. Sirtuin 7 (SIRT7) is a member of the sirtuin family of NAD+-dependent deacetylases mediating numerous biological processes. In this study, we detected the localization of SIRT7 in yak ovaries as well as explored the function of SIRT7 in yak CGCs. The results revealed that the SIRT7 protein was mainly localized in the cytoplasm of oocytes, granulosa cells and theca cells. The knockdown of SIRT7 in yak CGCs repressed cell proliferation and impacted the expressions of several apoptosis-related genes. Furthermore, oestrogen synthesis was also inhibited in SIRT7-deficient yak CGCs. The expressions of several sterogenesis-related genes decreased significantly following SIRT7 knockdown. In addition, the lack of SIRT7 in yak CGCs resulted in decreased levels of the TGFB/SMAD family members TGFB1, TGFBR1 SMAD2 and SMAD3. Moreover, the activation of the TGFB/SMAD pathway by adding TGFB/SMAD pathway activator SRI-011381 partially rescued the level of oestrogen secreted by SIRT7-deficient yak CGCs, as well as the expressions of steroidogenesis-related genes NR5A1 and CYP19A1. This research is the first to focus on the role of SIRT7 in yak ovary, and the outcomes offer new insights into the mechanism governing yak reproduction.
摘要译文
颗粒状颗粒细胞（CGC）是一种重要的卵巢体细胞，它具有与卵子发生，卵泡发育和胚胎发生有关的各种功能。关于CGCS的发展和功能的研究促进了对雌性动物生殖调节的理解。Sirtuin 7（SIRT7）是介导许多生物学过程的NAD+依赖性脱乙酰基酶的Sirtuin家族的成员。在这项研究中，我们检测到SIRT7在Yak卵巢中的定位，并探索了SIRT7在YAK CGC中的功能。结果表明，SIRT7蛋白主要定位于卵母细胞，颗粒细胞和theca细胞的细胞质中。YAK CGC中SIRT7的敲低抑制细胞增殖，并影响了几种与凋亡相关的基因的表达。此外，在缺乏SIRT7缺乏的YAK CGC中，雌激素合成也受到抑制。SIRT7敲低后，几个与静脉发育相关的基因的表达显着降低。此外，YAK CGC中缺乏SIRT7导致TGFB/SMAD家族成员TGFB1，TGFBR1 SMAD2和SMAD3的水平降低。此外，通过添加TGFB/SMAD途径激活剂SRI-011381激活TGFB/SMAD途径，部分地挽救了SIRT7缺乏YAK CGC分泌的雌激素水平，以及类固醇相关基因NR5A1和CYP19A1的表达。这项研究是第一个关注SIRT7在Yak卵巢中的作用的研究，结果为管理Yak繁殖的机制提供了新的见解。
Shaoshuai Gao[1];Liuqing Yang[1];Xue Yang[1];Yan Xiong[1];Wei Fu[1];Jian Li[1];Shi Yin[1]. Sirtuin 7 is essential for the survival and synthesis of oestrogen in yak (Bos grunniens) cumulus granulosa cells[J]. Reproduction in Domestic Animals, 2023,58(2): 323-332