摘要
BackgroundThe intestinal porcine enterocyte cell line (IPEC-J2) is a well-established model to study porcine intestinal physiology. IPEC-J2 cells undergo spontaneous differentiation during culture while changes in expression patterns of differentiated IPEC-J2 remain unclear. Therefore, this study was aimed to investigate the expression profiles of IPEC-J2 cells at the transcriptional level. Differentially expressed genes (DEGs), enriched pathways and potential key genes were identified. Alkaline phosphatase (AKP) and percentages of apoptotic cells were also measured.ResultsOverall, a total of 988 DEGs were identified, including 704 up-regulated and 284 down-regulated genes. GO analysis revealed that epithelial cell differentiation, apoptotic signaling pathway, regulation of cytokine production and immune system process, regulation of cell death and proliferation, cell junction complexes, and kinase binding were enriched significantly. Consistently, KEGG, REACTOME, and CORUM analysis indicated that cytokine responses modulation may be involved in IPEC-J2 differentiation. Moreover, AKP activity, a recognized marker of enterocyte differentiation, was significantly increased in IPEC-J2 after 14 days of culture. Meanwhile, annexin V-FITC/PI assay demonstrated a remarkable increase in apoptotic cells after 14 days of culture. Additionally, 10 hub genes were extracted, and STAT1, AKT3, and VEGFA were speculated to play roles in IPEC-J2 differentiation.ConclusionsThese findings may contribute to the molecular characterization of IPEC-J2, and may progress the understanding of cellular differentiation of swine intestinal epithelium.
摘要译文
背景技术肠猪肠细胞细胞系（IPEC-J2）是研究猪肠生理学的良好模型。 IPEC-J2细胞在培养过程中发生自发分化，同时差异化IPEC-J2的表达模式的变化仍不清楚。因此，本研究旨在研究转录水平的IPEC-J2细胞的表达谱。鉴定了差异表达的基因（DEGS），富集的途径和潜在的关键基因。还测量碱性磷酸酶（AKP）和凋亡细胞的百分比。鉴定了总共988℃，包括704个上调和284个下调基因。 GO分析显示，上皮细胞分化，凋亡信号通路，细胞因子产生和免疫系统过程的调节，细胞死亡和增殖，细胞结复合物和激酶结合的调节均显着富集。始终如一地，Kegg，反应和冠状分析表明，细胞因子响应调节可能参与IPEC-J2分化。此外，AKP活性是肠细胞分化的公认标记，在培养14天后IPec-J2显着增加。同时，膜蛋白V-FITC / PI测定显示培养14天后凋亡细胞显着增加。此外，提取10个轮毂基因，并推测STAT1，AKT3和VEGFA在IPEC-J2分化中发挥作用。结论STHESE发现可能有助于IPEC-J2的分子表征，并且可能进展对猪肠细胞分化的理解上皮。
Pi; Guolin[1];Song; Wenxin[1];Wu; Zijuan[1];Li; Yali[1];Yang; Huansheng[1]. Comparison of expression profiles between undifferentiated and differentiated porcine IPEC-J2 cells[J]. Porcine Health Management, 2022,8(1): 1-12