摘要
The study aimed to investigate the nutritional value of ramie (Boehmeria nivea) silage, and its consequences for chewing activity, rumen fermentation, and enteric methane (CH4) emissions in goats, by comparing it with corn stover (CS) silage. An in vitro ruminal experiment was firstly performed to investigate the substrate degradation and fermentation of CS and ramie silage. The ramie silage diet was formulated by replacing 60% of CS silage with ramie silage (dry matter (DM) basis). Eight female Xiangdong Black goats (a local breed in Southern China, 1 to 1.2 years of age) with BW of 21.0 ± 1.05 kg were used for this experiment and were randomly assigned to either one of the two dietary treatments in a cross-over design. The ramie silage had higher crude protein (CP) and ash content and lower hemicellulose content, together with decreased (p < 0.05) nutrient degradation and methane production and increased (p < 0.05) acetate molar percentage and acetate to propionate ratio through in vitro ruminal fermentation. Feeding the ramie silage diet did not alter feed intake (p > 0.05), decreased (p < 0.05) nutrient digestibility, and increased (p < 0.05) chewing activity and rumination activity, with reductions (p < 0.05) in eating activity and idle activity. Although feeding the ramie silage diet caused a greater (p < 0.05) molar percentage of acetate and lower molar percentage of propionate, it decreased the rumen-dissolved CH4 concentration and enteric CH4 emissions (p < 0.05). Feeding the ramie silage diet did not alter (p > 0.05) the population of bacteria, protozoa, and fungi; it increased the 16S rRNA gene copies of Ruminococcus flavefaciens (p < 0.05). Further 16SrRNA gene amplicon analysis indicated a distinct bacterial composition between the two treatments (p < 0.05). Feeding the ramie silage diet led to a lower abundance of genera Lawsonibacter, Sedimentibacter, Saccharofermentans, Sediminibacterium, and Bifidobacterium (p < 0.05). Ramie can be an alternative forage resource to stimulate chewing activity and reduce CH4 emissions in ruminants.
摘要译文
该研究旨在研究拉米（Boehmeria nivea）青贮饲料的营养价值，及其对山羊的咀嚼活性，瘤胃发酵和肠甲烷（CH4）排放的后果，通过将其与玉米臭味（CS）的青贮饲料进行比较。首先进行了体外瘤胃实验，以研究CS和拉米青贮饲料的底物降解和发酵。拉米青贮饲料饮食是通过用拉米青贮饲料（干物质（DM）基础）代替60％的CS青贮饲料来制定的。 BW为21.0±1.05千克的八只雌性江代黑山羊（中国南部的当地品种，1至1.2岁）用于该实验，并随机分配给交叉设计中的两种饮食治疗中的任何一种。拉米青贮饲料具有较高的粗蛋白（CP）和灰分含量和下半纤维素的含量，以及（p <0.05）的营养降解和甲烷产生（p <0.05）（p <0.05）乙酸盐摩尔百分比和乙酸盐的百分比和乙酸盐含量，并通过体外脉缪含量增加发酵。喂养拉米青贮饮食不会改变饲料摄入量（p> 0.05），减少（p <0.05）营养消化率，并增加（p <0.05）咀嚼活性和反省活性，饮食活动和闲置活动减少（p <0.05）活动。尽管喂食拉米青贮饮食会导致乙酸酸盐和丙酸较低的摩尔百分比较高（p <0.05），但它降低了瘤胃溶解的CH4浓度和肠肠排放量（p <0.05）。喂养拉米青贮饮食没有改变（p> 0.05）细菌，原生动物和真菌的种群。它增加了Ruminococcus flavefaciens的16S rRNA基因拷贝（p <0.05）。另外，16sRRNA基因扩增子分析表明两种治疗之间存在明显的细菌组成（p <0.05）。喂养拉米青贮饲料饮食会导致较低的劳森属，沉积物杆菌，糖果，sediminibacterium和双歧杆菌（p <0.05）。拉米可能是刺激咀嚼活动并减少反刍动物中CH4排放的替代草料资源。
Xu Tian;Cheng Gao;Zhengping Hou;Rong Wang;Xiumin Zhang;Qiushuang Li;Zhongshan Wei;Duanqin Wu;Min Wang. Comparisons of Ramie and Corn Stover Silages: Effects on Chewing Activity, Rumen Fermentation, Microbiota and Methane Emissions in Goats[J]. Fermentation, 2022,8(9): 432