摘要
This study aimed to investigate the effect of silage or dry cornstalk diets on methane emission, rumen fermentation, and methanogenic community, and reveal whether the change of methanogenic compositions was related to the methane production. A total of 39 sheep were divided into four groups, fed diets of different concentrate level based on silage or dry cornstalk roughage for 40 days. It was found that, at 20% concentrate level, the sheep fed silage could suppress methanogenesis significantly in contrast with the silage diet (p < 0.05). The ruminal acetate:propionate ratio was 3.17 in the silage-fed sheep significantly lower than 3.78 in the dry cornstalk-fed sheep (p < 0.05), reflecting the effect of fermentation on methane output was related to roughage types. Furthermore, the methanogens was found to be significantly lower abundance (p < 0.05), and showed a different pattern using multivariate statistical analysis in silage-fed sheep. Compared with dry cornstalk diet, silage diet of 20% concentrate reduced methane production, decreased methanogenic abundance, and induced change of Methanobrevibacter composition at strain levels. This study showed variation of methanogenic compositions at strain level and its probable relationship with methane production, and provided microbial information to explain the low methane output when the animals were fed silage.
摘要译文
本研究旨在探讨对甲烷排放，瘤胃发酵，产甲烷和青贮社区或干玉米秸秆饲料的效果，并揭示甲烷组合物的变化是否与甲烷产量。总共有39只绵羊分成四组，不同水平的集中基于青贮饲料或干玉米秸秆粗饲料40天饲喂。我们发现，在20％的浓缩物的水平，羊饲喂青贮饲料可以抑制甲烷与青贮饲料对比显著（P在青贮饲料喂养的羊比玉米秸秆干喂羊3.78显著降低17（P此外，甲烷被认为是显著低丰度（对干玉米秸秆饲料相比，20％的精矿减少甲烷生产青贮饲料，减少甲烷丰度和甲烷短组成的应变水平引起的变化。这项研究表明，在应变水平及其与甲烷产量可能关系甲烷组成的变化，和微生物提供信息，解释低甲烷输出，当动物饲喂青贮饲料。
Liu Chong[1]; Zhu Zhuping[1]; Guo Tongjun[2]; Luo Yongming[2] and Dong Hongmin[1,*];. Changes in methane emission, rumen fermentation, and methanogenic community in response to silage and dry cornstalk diets[J]. Journal of Basic Microbiology, 2014,54(6): 521-530