摘要
In cattle, leptin has been implicated in the control of ovarian function and has been shown to modulate steroid production by theca and granulosa cells in a number of species. However, a direct effect of leptin on bovine luteal function has not been demonstrated. This study was conducted to determine if the leptin receptor (OB-R) is expressed in the bovine corpus luteum (CL), and to examine the effects of leptin on progesterone production by dispersed luteal cells in vitro. RT-PCR was used to detect the presence of OB-R and, more specifically, the long, biologically active isoform (OB-Rb), in CL, collected on days 2–18 of the oestrous cycle (n = 18). The effects of leptin on progesterone production were investigated in dispersed luteal cells prepared from CL collected on days 5 and 8 (n = 14) of the cycle. The dispersed luteal cells were cultured for 24 hr with recombinant human leptin and/or LR3-IGF-1 and/or LH. OB-Rs, in particular, OB-Rb, were expressed in the CL at all stages of development. Progesterone production by luteal cells was increased (P < 0.001) by treatment with LH (10 ng/ml) but treatment with leptin alone had no effect. However, in the presence of IGF-1 (100 ng/ml), leptin (10 ng/ml) caused a significant (P < 0.005) increase in progesterone production. In conclusion, we have shown that the leptin receptor is expressed in the bovine CL and have demonstrated a modulatory effect of leptin on luteal progesterone production in vitro. Mol. Reprod. Dev. 74: 724–729, 2007. © 2006 Wiley-Liss, Inc.
摘要译文
在牛，瘦素有牵连的卵巢功能的控制，并且已显示调节类固醇生产由卵泡膜和颗粒细胞在许多物种。然而，瘦素对牛黄体功能的直接作用尚未得到证实。此研究的目的是确定是否瘦蛋白受体（OB-R）的表达在牛黄体（CL）并研究瘦素对孕激素分泌的影响，通过分散黄体细胞在体外。 RT-PCR法检测的OB-R的存在，更具体地，长，生物活性异构体（OB-Rb的），在CL，收集天发情周期2-18（N瘦素对孕激素分泌的影响从CL准备分散的黄体细胞收集在5天至8进行了调查（N分散的黄体细胞中培养24小时，用重组人瘦素和/或LR3-IGF-1和/或LH。 OB-Rs时，特别是，OB-Rb的，均在发展的所有阶段表示在CL。孕激素分泌黄体细胞显着升高（P在IGF-1的（100毫微克/毫升）中，瘦蛋白的存在下（10毫微克/毫升）引起显著（P我们已经表明，瘦蛋白受体表达于牛CL和已经证明瘦素对黄体孕激素生产的体外调节作用。摩尔。生殖。开发。 74：724-729，2007年。
L.T. Nicklin[1]; R.S. Robinson[1]; P. Marsters[2]; B.K. Campbell[2]; G.E. Mann[1] and M.G. Hunter[1,*];. Leptin in the bovine corpus luteum: Receptor expression and effects on progesterone production[J]. Molecular Reproduction and Development, 2007,74(6): 724-729