摘要
The baculovirus expression system (BES) has been one of the versatile platforms for the production of recombinant proteins requiring multiple post-translational modifications, such as folding, oligomerization, phosphorylation, glycosylation, acylation, disulfide bond formation and proteolytic cleavage. Advances in recombinant DNA technology have facilitated application of the BES, and made it possible to express multiple proteins simultaneously in a single infection and to produce multimeric proteins sharing functional similarity with their natural analogs. Therefore, the BES has been used for the production of recombinant proteins and the construction of virus-like particles (VLPs), as well as for the development of subunit vaccines, including VLP-based vaccines. The VLP, which consists of one or more structural proteins but no viral genome, resembles the authentic virion but cannot replicate in cells. The high-quality recombinant protein expression and post-translational modifications obtained with the BES, along with its capacity to produce multiple proteins, imply that it is ideally suited to VLP production. In this article, we critically review the pros and cons of using the BES as a platform to produce both enveloped and non-enveloped VLPs.
摘要译文
杆状病毒表达系统（BES）已经用于生产重组蛋白质需要多次翻译后修饰，如折叠的多用途平台之一，低聚化，磷酸化，糖基化，酰化，二硫键形成和蛋白水解切割。在重组DNA技术的进步已经促进了应用了BES的，并使得能够同时在单个感染表达多种蛋白质和以产生多聚体蛋白与它们的天然类似物共享功能相似。因此，在BES已用于重组蛋白的生产和病毒样颗粒的结构（VLP的），以及用于亚基疫苗，包括基于VLP的疫苗的开发。VLP上，它由一个或多个结构蛋白但没有病毒基因组，类似于真实的病毒粒子，但可以在细胞中不能复制。与BES得到，随着其容量的高品质的重组蛋白的表达和翻译后修饰，以产生多种蛋白质，意味着它非常适用于VLP生产。在这篇文章中，我们严格审查的优点和使用BES为平台，同时生产笼罩和无包膜病毒样颗粒的利弊。
Fuxiao Liu[a][b]; Xiaodong Wu[a]; Lin Li[a]; Zengshan Liu[b]; Zhiliang Wang[a]. Use of baculovirus expression system for generation of virus-like particles: Successes and challenges[J]. Protein Expression and Purification, 2013,90(2): 104–116